Rama
Badugu, Ph.D.
Application Support
Consultant
Roche Life Sciences
Tuesday
May 12, 2015
1: 00 pm ET
10: 00 am PT
DURATION
60 minutes
COST
Complimentary
Speakers
Jefrey S.
Buguliskis, Ph.D.
Technical Editor
GEN
Moderator
Increasing Throughput
in Protein Thermal
Shift Assays
Free Registration!
www.GENengnews.com/thermalshift
The screening of small molecule and ligand libraries that bind to protein
targets in a high-throughput manner is an important, if not time-consuming,
part of drug discovery. The process often requires screening thousands of samples
using a variety of assays over an extended period of time. Since protein targets
are susceptible to degradation and aggregation, time is of the essence in protein
stability screening.
Historically, protein interaction studies have been measured using an array
of techniques, including nuclear magnetic resonance, diferential scanning
calorimetry, and surface plasma resonance imaging. Additionally, protein melt
screening methods used in the past have been inefcient or expensive, capable
of analyzing only small number of samples at time or requiring large quantities
of protein samples, leading to increased costs per assay. Modern thermal shift
assays bridge the gap between time-consuming sensitive assays and high-
throughput screening platforms that are essential for the fast-paced nature
of drug library screening.
In this GEN webinar, Rama Badugu, Ph.D., Application Support Consultant
for Roche Life Sciences, will describe the use of thermal shift assays, also called
diferential scanning fuorimetry, for protein stability and ligand screening.
Furthermore, Dr. Badugu will highlight how these assays can be applied in
high-throughput screening manner using the Roche LightCycler® 1536
real-time PCR system.
A live Q&A; session will follow the presentation, ofering you a chance to pose
questions to our expert panelist.
You Will Learn
• The basics of thermal shift assays and how
they are applicable to drug discovery.
• The advantage of thermal shift assays over
traditional screening methods for protein-
protein or protein-ligand interactions.
• How to utilize the Roche LightCycler 1536
as a high-throughput screening apparatus
using the thermal shift assay technique.
Who Should Attend
• R&D; scientists
• Drug discovery researchers
• Investigators studying protein-protein
interactions
• High-throughput screening scientists
• Protein chemists
• Small molecule researchers
Webinars
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