Contents
www.GENengnews.com • Volume 35, Number 9 • May 1, 2015
News
6 Sticky Ends
8 Products & Services
10 Industry Watch
12 Discovery & Development
18 Genomics & Proteomics
24 Bioprocessing
32 Molecular Diagnostics
34 Best Science Apps
35 Clinical Trials
36 New Products
42 Calendar
42 Company Index
43 People
Tutorials
Assay Tutorial
16 Illuminating Protein:Protein
Interactions in Living Cells
Promega says that a bright donor and a
spectrally distinct acceptor can improve
the sensitivity and widen the range of a
bioluminescence-based technique.
OMICS Tutorial
22 Genotyping Using CGE
A new capillary gel electrophoresis system
from BiOptic promises plug-and-play DNA
fragment analysis, thanks to a pen-shaped
capillary gel cartridge.
Bioprocessing Tutorial
30 Manufacturing Methods
for Cell Therapies
Invetech discusses how equipment and
processing considerations for allogenic
therapeutics difer from those for autologous
therapeutics.
Quality, Yield Still Rule
Protein Production
In the land of bewilderingly diverse expression products, even
semi-uniform batchwork is king, whether the realm consists of
glycoforms, hemagglutinins, or antibody-drug
conjugates. PAGE 24
Could CRISPR be the
Magic Bullet?
In the shooting gallery that is the genome, you can be "on target"
and still fail to win a prize. Make sure that your CRISPR/Cas9 ammo
packs enough punch to make the desired edits. PAGE 1
Deciphering
Immune Cell Chatter
All is not quiet on the immunological front. Rat-a-tats along receptor
pathways and bursts from infammasomes and signalosomes are
louder than ever. Does the din mean a breakthrough is near? PAGE 12
FEATURES
Repositioning Old
Drugs for New Uses
The lovelorn are told that there's a lid for every pot. The same could
be said of jilted drugs, which are joining candidate databases, part
of pharma's new emphasis on matchmaking. PAGE 1
Columns
GEN Exclusive
7 Brain Projects Get
Researchers Thinking Big
Staggering after brains isn't just for zombies.
Just look at the neuroscientists mobbing around
the biggest "brain" of all, the connectome, through
BigNeuron and other projects.
Corporate Profle
10 Direct-from-Tissue
Bioanalytics
Cross a stream of laser-stirred particles and an
electrospray plume, says Protea Biosciences, and
your mass spectrometer can analyze molecules
from cells, tissues, and biofuids.
Bioprocessing Perspectives
28 Bring Mycoplasma
Under Control
With contaminated cultures costing us big-time,
the gold standard of mycoplasma detection—
end-of-process testing—is tarnished. But PCR-
based in-process testing has yet to shine.