Genetic Engineering & Biotechnology News

SEP15 2016

Genetic Engineering & Biotechnology News (GEN) is the world's most widely read biotech publication. It provides the R&D; community with critical information on the tools, technologies, and trends that drive the biotech industry.

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Target Engagement in Live Cells The NanoBRET™ Target Engagement (TE) Assay measures compound binding at select target proteins in live cells, in real time. The assay uses bioluminescence resonance energy transfer (BRET), achieved by transfer- ring the luminescent energy from NanoLuc ® luciferase to the fl uor-tagged ligand bound to the fused target protein. BRET makes it possible to directly measure compound permeability, binding affi nity and compound- target residence time. With the NanoBRET™ TE Assay you can: • Measure Target Engagement in Live Cells: Detect test compound binding affi nity and permeability in live cells. • Directly Measure Residence Time: Compound and tracer compete directly for the binding site. • Use Full-Length Protein: Assays rely on full-length proteins that are similar to the native proteins. • Assay HDACs, BET BRDs and kinases. To learn more visit: promega.com/ResidenceTime ©2016 Promega Corporation. All rights reserved. 29390100 Direct residence measurement of JQ1 at binding site on NanoLuc ® -BRD4 Fusion Protein. 13725MA 0 20 40 60 80 120 100 Time (minutes) Tracer only 1,000nM JQ1 with washout 20,000nM JQ1, no washout BRET Ratio (mBU) 0 10 20 30 40 Compound residence time measurement using NanoLuc ® -BRD4 Luciferase Fusion Protein.

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