Genetic Engineering & Biotechnology News

AUG 2017

Genetic Engineering & Biotechnology News (GEN) is the world's most widely read biotech publication. It provides the R&D community with critical information on the tools, technologies, and trends that drive the biotech industry.

Issue link: http://gen.epubxp.com/i/850756

Contents of this Issue

Navigation

Page 16 of 37

Single-Day RNA. Higher-quality, Thyroid FFPE RIN: 2.2 Dv200: 47% Lower-quality, Duodenum FFPE RIN: 2.5 Dv200: 29% Generate High-quality Libraries from Degraded Samples • Input as low as 25 ng of FFPE, depending on total RNA quality • Achieve low duplication rates and highly efficient, reproducible rRNA removal with degraded samples • Identify more unique transcripts and genes with equivalent sequencing Comparative Use with High- and Low-quality FFPE Samples Single-tube, Single-day Library Prep • Reduce hands-on and overall time through fewer enzymatic and reaction-cleanup steps • Produce strand-specific, sequencing-ready libraries from input RNA in approximately 4 hours • Choose between polyA selection, rRNA depletion or total RNA kits • Achieve higher throughput with an automation-friendly workflow Prepare sequencing-ready NGS libraries from 25 ng – 1 µg total RNA from human, mouse, or rat (HMR) species* * Data on file. For Research Use Only. Not for use in diagnostic procedures. © 2017 Roche, Inc. All rights reserved. Trademarks are the property of their respective owners. Introducing KAPA RNA HyperPrep Kits for NGS rRNA % Mapped duplication % 100 75 50 25 0 KAPA RNA HyperPrep with RiboErase TruSeq ® Stranded Total RNA with Ribo-Zero Gold NEBNext ® Ultra Directional with rRNA Depletion Percentage rRNA % Mapped duplication % 100 75 50 25 0 KAPA RNA HyperPrep with RiboErase TruSeq Stranded Total RNA with Ribo-Zero Gold NEBNext Ultra Directional with rRNA Depletion Percentage 22200 19700 17200 Genes detected 22200 19700 17200 Genes detected Transcripts detected KAPA RNA HyperPrep with RiboErase TruSeq Stranded Total RNA with Ribo-Zero Gold NEBNext Ultra Directional with rRNA Depletion 125000 105000 85000 KAPA RNA HyperPrep with RiboErase TruSeq Stranded Total RNA with Ribo-Zero Gold NEBNext Ultra Directional with rRNA Depletion 125000 105000 85000 Transcripts detected (Top) Fewer reads are wasted due to combined alignment to rRNA loci and PCR duplicates when using the KAPA RNA HyperPrep with RiboErase in comparison to competitor workflows. yroid libraries were generated using 25 ng inputs, and duodenum libraries were generated with a minimum of 100 ng, due to the lower quality of the starting material. (Bottom) More genes and transcripts were identified with the KAPA RNA HyperPrep with RiboErase (HMR) workflow in comparison to competitor workflows.

Articles in this issue

Links on this page

Archives of this issue

view archives of Genetic Engineering & Biotechnology News - AUG 2017