Genetic Engineering & Biotechnology News

AUG 2017

Genetic Engineering & Biotechnology News (GEN) is the world's most widely read biotech publication. It provides the R&D community with critical information on the tools, technologies, and trends that drive the biotech industry.

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8 | AUGUST 2017 | | Genetic Engineering & Biotechnology News Drug Discovery Feature Roundup The stars of live-cell imaging—the living cells—need pampering, just like the stars of stage and screen. If anything, the cells need more pampering. Otherwise, they may act abnormally, or even perish, during their performances. The problem is, cells caught in live-cell imaging's spotlight must endure unnatural conditions while continuing to act naturally. Fortunately, live-cell imaging has several ways it can ease the burden it places on cells. It can, for example, shoot "on location." That is, microscopy instrumentation can be made an integral part of an incubation system. Live-cell imaging can also limit phototoxicity, the harm caused when illumination is used to excite fluores- cent markers, by relying on efficient light capture, or by collecting images that are only as brilliant as necessary. Live-cell imaging, then, is often a balancing act. Besides trying to be sparing with light while still illuminating cellular processes, live-cell imaging may try to avoid jostling cells. Hence, the growing popularity of automated environmental controls and analytical software. Such controls can sustain cells while avoiding perturbing influences, and such software can keep track of cell movements and determine which pertain to cell function, and which may be nothing more than experimental artifacts. By incorporating advanced technologies, live-cell imaging may keep more of its projects on schedule and under budget, especially if high-throughput workflows are supported. It may even avoid stagey productions while embracing cinéma vérité approaches, capturing cell-level dramas such as differentiation and reprogramming, host-pathogen interactions, and the kinds of interplay unique to three-dimensional (3D) ensembles. By combining specific probes and high magnification, live-cell imaging can even delve into subcellular processes. To realize these visions, live-cell imaging needs more than its star players. It also needs star producers—auteurs capable of controlling all aspects of a collaborative work. That's the conclusion reached by our panel of experts. Representing some of the leading technology providers of live-cell imaging, these experts explain what is going on behind the scenes, and how academic and commercial laboratories are catching a new wave in the characterization of cellular processes. Lights! Camera! Live-Cell Imaging! E X P E R T P A N E L Peter Banks, Ph.D. Scientific Director, BioTek Instruments Daniel M. Appledorn, Ph.D. Director, U.S. Biology R&D, Essen BioScience Chris Shumate, Ph.D. President and CEO, Etaluma Patrick Schneider, Ph.D. Head of R&D, Technology and Business Development, MilliporeSigma Karin Boettcher, Ph.D. Associate Product Manager, Cellular Imaging and Analysis, PerkinElmer 8 | August 2017 | | Genetic Engineering & Biotechnology News

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