Genetic Engineering & Biotechnology News

SEP15 2017

Genetic Engineering & Biotechnology News (GEN) is the world's most widely read biotech publication. It provides the R&D community with critical information on the tools, technologies, and trends that drive the biotech industry.

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12 | SEPTEMBER 15, 2017 | GENengnews.com | Genetic Engineering & Biotechnology News Jonathan Weinreich Development of a single drug, whether it is a new chemical entity, biotherapeutic, or genetic/cellular therapy, requires signifi- cant investment of resources. Each step of the process—from early discovery through production and delivery—must be fully ex- plored, characterized, and understood to prevent issues resulting from cell stress, cell death, protein aggregation, and factors af- fecting reliable manufacturing of the drug. ELISAs (enzyme-linked immunosorbent as- says) are well suited for contamination mon- itoring in bioprocess applications. Briefly, ELISAs utilize a matched antibody pair to detect the presence of a specific analyte. In a typical ELISA, a 96-well plate is coated with a capture antibody. The researcher then incubates the wells with the target— some wells receive a known concentration, while others receive samples with unknown concentration. Then, a detector antibody is added, and an enzyme is added to develop color. The optical density (OD) of each well is read at a specific wavelength, and using the OD of the wells of known concentra- tion, researchers can interpolate unknown concentrations. ELISAs are fully quantita- tive and sensitive, with kits on the market able to detect down to the picogram-per- mL level. Whether one works in drug discovery, up- stream, or downstream bioprocessing, there exists a range of ELISAs to help research- ers maintain cell-line viability, optimize and monitor product integrity, and maximize yield. In this tutorial, the authors will intro- A Go-To Assay for Contaminant Detection Back to Basics: Using ELISA Kits for Bioprocess Optimization Drug Discovery Tutorial Figure 1. Assay recognition of different Protein A constructs, post boiling. Resulting concentrations were interpolated from kit standard curve. Percent recovery was calculated by dividing observed concentration by expected concentration. (A) Natural Protein A from Staphylococcus aureus, n=9. (B) Recombinant Protein A from Escherichia coli, n=9. (C) Recombinant Cys-Protein A from E. coli, n=12. (D) Recombinant alkaline-resistant Protein A variant from E. coli, n=12. Graphical data represent statistical mean ±1 standard deviation. VIAFLO II ASSIST VOYAGER II Adjustable Tip Spacing Pipette Motorized tip spacing enables parallel transfers of multiple samples between labware of different sizes and formats. The tip spacing can be changed by the simple push of a button, no manual adjustments or two handed operations are needed. ARE YOU PIPETTING SAMPLES BETWEEN DIFFERENT LABWARE FORMATS? www.integra-biosciences.com EVOLVE VIAFLO 96 I 384

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