Genetic Engineering & Biotechnology News

SEP15 2017

Genetic Engineering & Biotechnology News (GEN) is the world's most widely read biotech publication. It provides the R&D community with critical information on the tools, technologies, and trends that drive the biotech industry.

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©2017 Promega Corporation. All rights reserved. 38587242 www.promega.com/ realtimeApoptosis Plate-based, non-lytic assay Scalable to 384 and 1536 wells Simple "add-and-read" method Monitor cell state continuously within a single sample well using the RealTime-Glo ™ Annexin V Apoptosis and Necrosis Assay . Annexin V:phosphatidylserine binding is detected with a simple luminescence signal, and secondary necrosis with a fluorescent signal. This real-time assay is perfect for confirming apoptosis and can be multiplexed with other assays to gain a complete picture of cell health. 0 16 8 24 32 40 48 0 70,000 140,000 210,000 280,000 350,000 2,000 4,000 6,000 8,000 10,000 Time (hours) Luminescence (RLU ) Fluorescence (RFU) K562 Cells: 1.1µM Bortezomib Intrinsic Inducer of Apoptosis 8 hours 20 hours ~ 12 hours Fluorescence (DNA staining) Luminescence (Annexin V:PS binding)

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