Genetic Engineering & Biotechnology News

OCT15 2017

Genetic Engineering & Biotechnology News (GEN) is the world's most widely read biotech publication. It provides the R&D community with critical information on the tools, technologies, and trends that drive the biotech industry.

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Vicki Glaser Analysts at Technavio have predicted the global hu- manized mouse model market to grow at a compound annual growth rate (CAGR) of almost 10% during 2017–2021, attributing this growth to the increasing number of applications in fields including oncology, immunology, neuroscience, and toxicology. In oncol- ogy, the emerging success of immunotherapies such as antibody-based checkpoint inhibitors is driving de- mand for accurate, more predictive preclinical animal models. Both syngeneic and humanized models have valuable roles in immuno-oncology drug discovery and development, and it is important to understand the advantages, Cancer Immunotherapy Spurs Work on Better Animal Models Single-cell genomics helps obtain and write those com- plex biographies, yielding insights into normal cell dy- namics, and provides information about when cells go rogue and turn into tumors. Single-cell genomics, a field still in its infancy, cap- tures genome-wide data at the resolution of individual cells. With its ability to depict thousands to millions of individual cells at once, the potential is enormous for shedding new light on fields such as immunology, neu- rology, and cancer. However, a common challenge of heterogeneous systems is that they often contain mas- sive numbers of cells. For example, a centimeter-sized tumor can consist of hundreds of millions of cancer cells. Additionally, each cell has only a tiny bounty of DNA, making it a challenge to accurately amplify and sequence individual cells. Despite these limitations, innovative technologies and platforms are being developed to not only enhance throughput, but also to quickly evaluate the vast datasets generated. Recent meetings, such as the Precision Medi- cine World Conference and SelectBio's RNA-Seq, Single Cell Analysis & Single Molecule Analysis conference, present new forums to discuss this young and exciting field. Some approaches are employing microfluidics in the form of nanodrops to encase cells or microfluidics chips with gel beads containing bar-coded oligonucleotides. An- other emerging field is the use of cell nuclei for single nu- clei RNA-seq. The latter can avoid the harsh treatment to separate or lyse whole cells that have the potential to alter gene expression. Also on the horizon are computational pipelines and visualization tools for effi- ciently processing million-cell datasets. see page 14 Kathy Liszewski Like snowflakes, fingerprints, and retinas, every cell has a unique personality. Whether wandering through the blood- stream as solitary nomads or gregariously congregating in tissue, each cell has a unique story to tell. The Rise of Single- Cell Genomics October 15, 2017 Bioprocess Control Specialist Focuses on Single- Use Technology 6 Copy Number Variation Probes Inform Diverse Applications 8 Examining Inflammatory Cytokines in Serum and Plasma 16 see page 26 Angelo DePalma, Ph.D. Authentication is becoming a hot-button topic among researchers. For example, researchers who use cells that are meant to possess distinct characteristics have been making an issue of cell-line authentication. 1 With increas- ing frequency, researchers who use antibodies are raising authentication concerns of their own. These researchers are insistent that antibodies be val- idated for function and purpose. Antibody Characterization Balances Rigor and Reason see page 20 Leading the Way in Life Science Technologies Sequencing DNA one cell at a time helps scientists discern the true nature of the heterogenous milieu that comprise various tissues. Blueringmedia/Getty Images The structure of antibodies is well known, but understanding their true function is where things get more nebulous. knorre/Getty Images

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