Genetic Engineering & Biotechnology News

OCT15 2017

Genetic Engineering & Biotechnology News (GEN) is the world's most widely read biotech publication. It provides the R&D community with critical information on the tools, technologies, and trends that drive the biotech industry.

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Genetic Engineering & Biotechnology News | GENengnews.com | OCTOBER 15, 2017 | 23 The CytoSMART™ 2 System, recently intro- duced by Lonza, is designed for bright-field live-cell imaging for applications such as gen- eral cell culture monitoring, documentation, and scratch/migration assays, which require a larger field of view. GEN recently spoke with Claudia Schwartz, product manager, CytoSMART, to learn more about the product. GEN : What's different about the updated version compared with the first version? Ms. Schwartz: The CytoSMART 2 Device features an advanced optical system and more powerful camera unit, which enables researchers to capture images of their cell cultures at a higher resolution, compared with the original CytoSMART System. Ad- ditionally, images taken can now be digitally enlarged two-fold to display more cellular detail for enhanced monitoring of cell cul- tures, migration assays, and differentiation experiments. Data transfer from the CytoSMART to the cloud used to be transmitted via WiFi. CytoSMART 2 transfers data via WiFi or LAN transmission. Combined with its ability to operate offline and its flexibility in image recording and storage, the system seamlessly integrates with different network set-ups, pro- viding a truly flexible solution to laboratories. CytoSMART 2 is operated via a CytoSMART Tablet that can be controlled by either the on-screen keyboard or the new keyboard base attached to it. The keyboard lets scientists operate the CytoSMART 2 Sys- tem in an environment where wearing gloves is mandatory. This update was made to better meet the needs of scientists in the laboratory. GEN : How does the new system work? Ms. Schwartz: CytoSMART 2 consists of a CytoSMART 2 Device and a tablet and key- board. The device is placed in the incubator and operated via the tablet from the outside. Once CytoSMART 2 is connected to the CytoSMART Connect Cloud via WiFi or LAN transmission, recordings (referred to as CytoSMART Projects) can be started by entering project details, such as the user's email address, experiment name, and re- cording interval. Taken images are transmitted to the Cloud, where they are processed. When start- ing a project, the user immediately receives an email with the access link to view the data on the CytoSMART Connect Cloud via comput- er, tablet, or smart phone. As soon as the user stops the project re- cording, all data can be downloaded from the CytoSMART Connect Project Page, such as images (.jpg files), time-lapse video (.avi files) or temperature and confluency data (.csv files for further processing in Excel). Alternatively, CytoSMART 2 can operate offline. In this case, recorded images are saved locally on the tablet. GEN : What new possibilities does CytoSMART 2 open for researchers? Ms. Schwartz: CytoSMART 2 is designed to support scientists with a range of appli- cations. By doing this, the system benefits biopharmaceutical firms and academic re- searchers who are undertaking cell culture monitoring and cell culture standardiza- tion. The applications CytoSMART 2 is suited for are: • Video recording and documentation of migration assays. For example, scratch assays, tube formation assays, chemotaxis, and 3D-cell invasion assays. We have a white paper on the CytoSMART System and migration assays that provides a lot of useful information on this particular appli- cation. • Cell culture standardization by auto- matic confluency determination and alert functionality notifies scientists when cultures have reached a desired confluency without having to step into the laboratory. While not always easy to maintain, standardized cell culture is of great importance as the condi- tion of a cell culture may have a significant influence on the downstream results obtained with the cultured cells. One way to obtain a more standardized cell culture is to use cells for any downstream assay at consistent confluency. However, the determination of cell confluency is very user dependent. CytoSMART 2 overcomes the issue of biased determination of cell culture confluency in offering a user-independent analysis of the cell confluency, which is re- corded in the CytoSMART Project. n To read an extended version of this article please go to www.GENengnews.com, place your cursor on MAGAZINE at the top of the page, and then click on CURRENT ISSUE. WE BRING LIFE TO YOUR LABORATORY. Explore & get your free demo at www.infors-ht.com/eve Shakers | Bioreactors | Bioprocess Platform Software www.infors-ht.com Connect and manage different systems. One single solution to unify all your equipment and data. eve ® – the revolutionary bioprocess platform software. © Space Telescope Science Institute Lonza Upgrades Live-Cell Imaging System Perspectives Bioprocessing Lonza officials note that the CytoSMART 2 System can take time-lapse recordings without having to remove cultures from the incubator.

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