Genetic Engineering & Biotechnology News

DEC 2017

Genetic Engineering & Biotechnology News (GEN) is the world's most widely read biotech publication. It provides the R&D community with critical information on the tools, technologies, and trends that drive the biotech industry.

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CAR-T Cell Target Selection Using Innovative in Situ Hybridization Technology Chimeric antigen receptor T (CAR-T ) cells are promising treatments for hematologic and solid malignancies. CAR-T cells can recognize and eliminate neoplastic cells expressing specific protein antigens. However, the current generation of CAR-T cells cannot distinguish between neoplastic and normal cells that may also be expressing the same antigen, thus potentially resulting in "on-target/off-tumor" toxicity. In this GEN webinar, we will review the RNAscope® in situ hybridization (ISH) method and show applications of the technology in the CAR-T cell and immuno-oncology fields. We will demonstrate how RNAscope ISH can be utilized to identify novel CAR-T cell targets and subsequently qualify monoclonal antibodies directed against those targets for immunohistochemistry. We will show how ISH can be used to predict CAR-T cell target organ toxicity in preclinical mod- els. Finally, we will discuss optimal preparation of tissues, cell pellets, and cytospin slides for evaluation by ISH. A live Q&A session will follow the presentation, offering you a chance to pose questions to our expert panelists. Free Registration! View It Now! On Demand DURATION: 60 minutes COST: Complimentary Speakers James B. Rottman, D.V.M, Ph.D., D.A.C.V.P. Senior Director of Translational Development Bluebird Bio Courtney Anderson, Ph.D. Group Leader, Applications Advanced Cell Diagnostics Produced with support from Webinars You Will Learn • How RNAscope ISH technology can streamline your target identification and selection workflows • The advantages of using ISH for immunotherapeutic target cell selection • How ISH evaluation of target expression in normal tissues can be used to predict CAR-T cell– induced toxicity • Optimal tissue, cell pellet, and cytospin slide preparation for RNAscope ISH

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