Genetic Engineering & Biotechnology News

NOV1 2018

Genetic Engineering & Biotechnology News (GEN) is the world's most widely read biotech publication. It provides the R&D community with critical information on the tools, technologies, and trends that drive the biotech industry.

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Global Flow Cytometry Market $3.09 billion 2016 $4.79 billion 2022 Source: (CAGR 7.8%) November 1, 2018 "If I were to study a human population, I could distinguish males and females—two subpopulations—but in terms of who was suffering diabetes, you can only see the difference when you get a closer look," says Akos Vertes Ph.D., professor of chemistry and professor of biochemistry and molecular biology at George Wash- ington University. Dr. Vertes will be one of the speakers at the 6th Annual Single- Cell Analysis Congress, part of the Genomics and Synthetic Biol- ogy Series UK, scheduled for November 8–9. Among the topics to be covered are the use of fluorescence microscopy and micro- fluidics to classify cancer and rare diseases, and to Basing Prognoses on Measures of Immune Cell Morphology Joseph Krueger, Ph.D., CSO of Flagship Biosciences, discussed the creation of predictive diagnostics for mea- suring tumor-infiltrating lymphocytes (TILs) and a more contextual approach to marker assessments. Studies in multiple tumor types have documented that the presence and the quantity of TILs strongly cor- relate with increased survival. But methodological chal- lenges in obtaining morphological measures have lim- ited the usefulness of TILs in standard prognostic panels and patient profiles. Presently, TIL counts are primarily measured by immunohistochemistry (IHC). A major limitation of IHC is the 2D nature of stained slides. Assaying a 2D cross-section of a 3D tumor severely limits the infor- mation sampled and increases variation. A second key shortcoming of IHC is the difficulty of standardization among laboratories. Staining intensity and specificity vary significantly from lab to lab even when the same antibodies are used with a shared operating procedure. Flagship Biosciences' Computational Tissue Analy- sis (cTA) platform provides an alternative approach for solving the key problems of variability, lack of precision, and insufficient clarity in the relationship between test results and clinical outcomes. Dr. Krueger explained that Flagship developed the cTA platform to simplify tissue context biomarker analysis and to determine the cutoff values that define a positive result. "The cTA plat- form uses proprietary com- puter algorithms to quantify The Scoop Top 10 U.S. Biopharma Clusters 6 Biomanufacturers Take the Heat off Their Cell Lines 16 Flow Cytometry Breaks with Tradition 14 Microfluidic Dragnet Catches Most-Wanted Antibodies 8 Do CRISPR Risks Outweigh Rewards? 29 see page 26 see page 10 Vivienne Raper, Ph.D. Single-cell analysis is growing in popularity. Visualizing processes within individual cells, whether using microscopy or creating maps of gene expression, is garnering a deeper understanding of how cells within the same tissue may behave differently from one another, despite appearing similar on first glance. Patricia Fitzpatrick Dimond, Ph.D. At the Clinical Biomarkers and World CDx Conference, held recently in Boston, industry and academic scientists convened to address approaches to enhancing and validating the next generation of predictive biomarkers and promoting the adoption of high-value precision medicines and diagnostic testing worldwide. Unravelling the Secrets inside Single Cells At Bar-Ilan University, Yaron Shav-Tal, Ph.D., and colleagues have combined fluorescence lifetime imaging microscopy (FLIM) and fluorescence resonance energy transfer (FRET) to track mRNA as it travels though nuclear pores. The team obtained this image, which shows nuclear pores (red), TAP export receptor complexes (green), and DNA (blue). Yaron Shav-Tal, Ph.D. Precision Biomarkers and Medicines Go Global

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