Speakers
Petr Jandik
Principal Scientist
Research and
Development
Thermo Fisher Scientifc
Analysis of N-Glycan Released
from Biopharmaceutical
Glycoproteins Using
HPAE-PAD
Sebastian
Kandzia
Webinars
Head of
Oligosaccharide
Analytics
GlycoThera
Moderator
Free Registration!
www.GENengnews.com/HPAE
Unlike conventional drugs, recombinant protein drugs are highly
John Sterling
Editor in Chief
GEN
complex molecules with remarkable heterogeneity. Protein glycosylation
What You Will Learn
n
Principles of HPAE-PAD and how it can be a
powerful tool in carbohydrate analysis
n
Anion-exchange columns optimized for
carbohydrate separations
n
GMP validated HPAE-PAD methods according to
ICH/EU guidelines for release testing of human
biopharmaceuticals
n
HPAE-PAD mapping of native N-glycans released
from recombinant biopharmaceuticals e.g. EPO, FSH
and Erbitux
afects the safety, efcacy, and serum half-life of these biopharmaceuticals.
Therefore analysis of the glycan pattern is an important issue for
characterization and quality control in the biopharmaceutical industry.
When making these products, the manufacturers have to deliver a consistent product quality to guarantee a reproducible clinical performance.
Underivatized N-glycans have no light-absorbing properties. They
have to be derivatized with a chromaphore (2-aminobenzamide, 2-AB)
in order to be analyzed by HPLC and UV detection. High performance
anion exchange chromatography with pulsed amperometric detection
(HPAE-PAD) does not require modifcation of the glycans before analysis.
Who Should Attend
n
Analytical chemists who want additional understanding
of HPAE-PAD and carbohydrate analysis
n
Product development scientists who need to
become knowledgeable about methods for studying
and identifying carbohydrates in biopharmaceuticals
n
Quality control and regulatory afairs professionals
in the biotechnology industry
This technique is optimized to determine monosaccharides, sialic acids,
oligosaccharidesand other carbohydrate components of glycoproteins.
This webinar will discuss how HPAE-PAD performs accurate and
consistent mapping of N-glycans released from biopharmaceuticals
such as EPO, FSH and Erbitux.
DATE
View It Now!
On Demand
DURATION
60 minutes
COST
Complimentary
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