Genetic Engineering & Biotechnology News

MAY1 2015

Genetic Engineering & Biotechnology News (GEN) is the world's most widely read biotech publication. It provides the R&D community with critical information on the tools, technologies, and trends that drive the biotech industry.

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Rama Badugu, Ph.D. Application Support Consultant Roche Life Sciences Tuesday May 12, 2015 1: 00 pm ET 10: 00 am PT DURATION 60 minutes COST Complimentary Speakers Jefrey S. Buguliskis, Ph.D. Technical Editor GEN Moderator Increasing Throughput in Protein Thermal Shift Assays Free Registration! The screening of small molecule and ligand libraries that bind to protein targets in a high-throughput manner is an important, if not time-consuming, part of drug discovery. The process often requires screening thousands of samples using a variety of assays over an extended period of time. Since protein targets are susceptible to degradation and aggregation, time is of the essence in protein stability screening. Historically, protein interaction studies have been measured using an array of techniques, including nuclear magnetic resonance, diferential scanning calorimetry, and surface plasma resonance imaging. Additionally, protein melt screening methods used in the past have been inefcient or expensive, capable of analyzing only small number of samples at time or requiring large quantities of protein samples, leading to increased costs per assay. Modern thermal shift assays bridge the gap between time-consuming sensitive assays and high- throughput screening platforms that are essential for the fast-paced nature of drug library screening. In this GEN webinar, Rama Badugu, Ph.D., Application Support Consultant for Roche Life Sciences, will describe the use of thermal shift assays, also called diferential scanning fuorimetry, for protein stability and ligand screening. Furthermore, Dr. Badugu will highlight how these assays can be applied in high-throughput screening manner using the Roche LightCycler® 1536 real-time PCR system. A live Q&A session will follow the presentation, ofering you a chance to pose questions to our expert panelist. You Will Learn • The basics of thermal shift assays and how they are applicable to drug discovery. • The advantage of thermal shift assays over traditional screening methods for protein- protein or protein-ligand interactions. • How to utilize the Roche LightCycler 1536 as a high-throughput screening apparatus using the thermal shift assay technique. Who Should Attend • R&D scientists • Drug discovery researchers • Investigators studying protein-protein interactions • High-throughput screening scientists • Protein chemists • Small molecule researchers Webinars Produced with support from

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