Genetic Engineering & Biotechnology News

OCT1 2012

Genetic Engineering & Biotechnology News (GEN) is the world's most widely read biotech publication. It provides the R&D; community with critical information on the tools, technologies, and trends that drive the biotech industry.

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New Products For more information, visit our website (genengnews.com) and click on New Products. There you'll be able to see more products from industry leaders and directly email suppliers for more information. Whole-Genome Amplification Kit illustra™ Ready-To-Go™ GenomiPhi™ kits provide researchers with a predispensed, room temperature–stable formulation for whole-genome amplification, enabling a workflow for obtaining large amounts of high-quality DNA from small genomic DNA samples. The new kits incorporate Ready-To-Go stabilization technology, which can deliver single-dose reaction mixes in a solid format that can be stored for months at the bench without the need for re- frigeration. illustra Ready-To-Go GenomiPhi kits can be used to amplify DNA from blood lysates and dried blood spots. GE Healthcare Life Sciences www.gelifesciences.com DNA Polymerase The Q5™ high-fidelity DNA polymerase can provide high- fidelity amplification while also offering amplification across a range of amplicons, including those that are GC- and AT-rich. This polymerase is fused to the processivity- enhancing Sso7d DNA binding domain, which can improve fidelity, speed, and performance reliability (am- plifying targets up to 20 kb long with extension times as low as 10 seconds per kb). New England Biolabs www.neb.com Assay Plate Stem Cell Culture Medium TeSR™-E8™ is a feeder-free culture medium for human embryonic stem cells (ES cells) and human induced pluripotent stem cells (iPS cells). TeSR- E8 contains only the essential eight components required for mainte- nance of ES cells and iPS cells. TeSR-E8 can be used with a surface coating of BD Matrigel™ hESC-qualified Matrix or vitronectin. Stemcell Technologies www.stemcell.com The 96-well cellular label-free as- say plate for the EnSpire® label-free multimode plate reader platform is designed to support basic life science research as well as assay development applications. The plate requires no assays; each well of the microplate contains an optical sensor, so work- flows require only the sample, microplate, and reader. When used with the EnSpire platform, the assay plate series enables researchers to measure phenotypic changes, and observe pharmacology comparable to that of labeled assays, but with richer data output. The integrated cellular response obtained from a label-free assay enables characterization of the signaling pathways involved, which can be af- fected by biased agonism, dimerization, and allosterism. PerkinElmer www.perkinelmer.com Image Analysis Flow Cytometry Laser A 561 nm laser option is now available for new 561 nm laser-ready Gallios flow cytom- eters. The new laser option can aid researchers in analysis of a range of fluorescent proteins and excitement of PE/PE tandems. Good excitation is offered for red fluores- cent proteins such as mCherry and DsRed. Yellow-green excitation provided at 561 nm can produce less autofluorescence in cells, increasing the signal-to-noise ratio for PE and DsRed detection. There is nearly no crossover contamination in the green detec- tion channel, allowing simultaneous fluorescein or GFP detection without blocking optics. The new laser is externally coupled through an optical fiber and can be field installed on new Gallios 561 nm laser-ready analyzers. Beckman Coulter www.beckmancoulter.com System The PXi is a high-resolution, multi-application image analysis system for scientists who need a compact, one- click method for imaging chemiluminescent and fluorescent blots, as well as 1D gels (up to 10 cm x 12 cm) stained with any type of fluo- rescent dye. The compact PXi can image faint bands with its 6.3 million-pixel camera and large fixed aperture lens. One can fit a range of lighting including IR lighting and filter options inside the PXi system, and using GeneSys imaging software, the PXi can be set up to automatically select good conditions. Syngene www.syngene.com 82 | October 1, 2012 | genengnews.com | Genetic Engineering & Biotechnology News

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