Genetic Engineering & Biotechnology News

JAN15 2018

Genetic Engineering & Biotechnology News (GEN) is the world's most widely read biotech publication. It provides the R&D community with critical information on the tools, technologies, and trends that drive the biotech industry.

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50% OFF SINGLE-DAY RNA Try KAPA RNA HyperPrep with half-price, 24-reaction sample kits until 3/31/18.* KAPA RNA HyperPrep Kits utilize novel chemistry that enables the combination of enzymatic steps and fewer reaction purifications. • Streamlined, single-day RNA library construction • Greater success with low-input and degraded RNA samples • Robust performance to detect more transcripts Qualifying trial kits: • KAPA RNA HyperPrep Kit (24 rxn) • KAPA mRNA HyperPrep Kit (24 rxn) • KAPA RNA HyperPrep Kit with RiboErase (HMR) (24 rxn) • KAPA Dual-Indexed Adapter Kits (96 adapters) Contact your local Roche sales representative for a quote or enter promo code PRO17D002S at: Differentially expressed transcripts (Fold change ≥2; p≤0.001) KAPA RNA HyperPlus libraries identify more differentially expressed transcripts. Using 100 ng of matched breast normal and tumor RNA, libraries were generated in duplicate according to manufacturers' recommendations. Overlap analysis revealed that the Kapa workflow identified the majority of transcripts identified by Illumina ® and/or NEB, plus an additional 299. Of the transcripts identified by Illumina or NEB, but not by Kapa, only 2 transcripts are protein-coding. In contrast, of the 299 transcripts identified by Kapa only, 174 are protein coding. 299 42 21 (1 protein coding) 18 (1 protein coding) 2 (0 protein coding) 15 36 (174 protein coding) KAPA RNA HyperPrep Kit with RiboErase (HMR) TruSeq Stranded Total RNA Library Prep with Ribo-Zero Gold NEBNext Ultra Directional Library Preparation with the rRNA Depletion Kits Data on file. For Research Use Only. Not for use in diagnostic procedures. *Discount applies to list price, and may not be combined with other offers. Valid for the purchase of one 24-reaction RNA HyperPrep Kit and one Dual-Indexed Adapter Kit. Limit one redemption per lab; new US-based customers only. Offer expires March 31, 2018. © 2018 Roche Sequencing. All rights reserved. KAPA is a registered trademark of Roche. All other trademarks are the property of their respective owners. More transcripts, greater flexibility, less sequencing

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