Genetic Engineering & Biotechnology News

MAY15 2018

Genetic Engineering & Biotechnology News (GEN) is the world's most widely read biotech publication. It provides the R&D community with critical information on the tools, technologies, and trends that drive the biotech industry.

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Genetic Engineering & Biotechnology News | | MAY 15, 2018 | 11 uid chromatography (UHPLC) system. The UHPLC system capable of operat- ing at inlet pressures of up to 40,000 psi and was coupled online to a high-resolution mass spectrometer. The technical limit of pressure was addressed through a special arrange- ment of pumps and valves. Existing technol- ogy has allowed pumps to operate as high as 100,000 psi; however, this technology is not suitable for gradients. A gradient pump was used to form a gra- dient at low pressures, and then the stored gradient was driven using the very high pres- sure pump. Certain key components, valves and fittings that can withstand the high pres- sures, were invented by a collaborator. The system has been used for initial test analyses of metabolomic and lipidomic samples. According to Dr. Kennedy, it is pro- ducing clearly sharper peaks, which in turn should mean more resolved peaks, as com- pared to a commercial capillary LC system. It remains to be determined if the system af- fects the information output of an undirect- ed metabolomics experiment. More study is needed. A lot remains un- known about packing columns to get the theoretical efficiencies possible with high pres- sures and finding the sweet spot for column and particle dimensions. Although efficiencies may be predicted with theory, they depend on how well the columns are packed. In addition, the plumbing of the system is not yet ideal for compounds that are not well retained. The dead-time peaks can be broad; further investigation is required to see if this complication can be eliminated. Dr. Kennedy also plans to test more complex mixtures of peptides and proteins to see if the proteomics will be improved. YOU ARE NOT A ROBOT... SO DON'T ACT LIKE ONE FREE YOURSELF FROM ROUTINE PIPETTING Making hands-free serial dilutions, reagent additions and sample reformatting very affordable for every lab. Compatible with all Integra's electronic pipettes from 4 to 16 channels for consistent results and unbeatable ergonomics. VOYAGER - Adjustable Tip Spacing Pipettes VIAFLO - Electronic Pipettes ASSIST PLUS Automating Multichannel Pipettes Drug Discovery Isotopic ratio outlier analysis (IROA) was used by University of Florida researchers to generate this tryptophan analysis, which shows how the 95% 13 C-labeled sample provides an n−1 isotope pattern starting at 216.1345. Peaks for the labeled sample (purple shading) are compared to peaks for the unlabeled standard (tan shading). Developed by scientists at the University of Michigan, the UHPLC system shown in this image can operate at pressures as high as 40,000 psi. The gradient pump (upper center) creates a mobile phase gradient and delivers it to the gradient loop (bottom left). The pneumatic amplifier pump (bottom center) then drives the fluid into the column (upper left) at high pressures. Flow is controlled by freeze-thaw valves (bottom left). The column is interfaced to a quadrupole time-of-flight MS (right).

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