Genetic Engineering & Biotechnology News

MAY15 2018

Genetic Engineering & Biotechnology News (GEN) is the world's most widely read biotech publication. It provides the R&D community with critical information on the tools, technologies, and trends that drive the biotech industry.

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Genetic Engineering & Biotechnology News | | MAY 15, 2018 | 27 For more information, visit our website (, click on New Products, and search through our comprehensive new products database. New Products Antibody Labeling Lightning-Link ® enables the direct labeling of antibodies or proteins in as little as 20 minutes, for use in R&D applications, drug discovery, and the development of diagnostic kits. Lightning- Link kits require only 30 seconds hands-on time, and there are no separation steps involved— meaning 100% of materials are retained. The company's increased manufacturing capability means the Lightning-Link product range is now available in volumes from 10 µg to 100 mg, in single-reaction vials tested with Rabbit IgG in ELISA. For antibody manufacturers that have fixed internal processes for conjugation, bulk supply of ultra-high-quality raw materials and labels that are used in the Lightning-Link kits are also available. Expedeon Light-Sheet Imaging Microscope The ct-dSPIM is a flexible and easy-to-use Selective Plane Illumination Microscopy (SPIM) configura- tion made from flexible and modular microscope components, allowing dual-views of large samples of cleared tissue. When used with ASI's and Special Optics's new objective optimized for light-sheet im- aging of cleared tissue, imaging depth can exceed 5 mm into flat samples. The objective accommodates media RI from 1.33 to 1.56 (aqueous or organic me- dia). The ct-dSPIM has successfully imaged various cleared tissue samples from microtome-cut slices to whole mouse brains. Unlike other light sheet techniques, this SPIM concept uses two objectives, oriented 45° from vertical, at right angles to one another above a horizontally mounted sample in an open dish. A light sheet is created from one objective and imaged through the other. A stack of images can be collected by moving the light sheet through the sample. In the case of the ct- dSPIM, the sample is normally moved through a stationary light sheet using an XY stage. Applied Scientific Instrumentation lncRNA Workflow The lncRNA Workflow is an RT-qPCR workflow that is optimized for highly sensitive and specific quantification of long noncoding RNAs (lncRNAs) for gene expres- sion analysis. The workflow provides a streamlined, cost-effective alternative to RNA-seq for ln- cRNA discovery and validation. Quantification of lncRNAs is particularly difficult due to low expression levels seen in some developmental contexts or with certain cell types. Furthermore, lncRNA primer de- sign requires considerable time and effort as targets are often positioned in complex genomic loci and can form secondary structures. PrimePCR lncRNA assay designs were developed in collaboration with transcriptome experts at BioGazelle using a design algorithm specifically built for lncRNAs. The lncRNA Workflow solves the most pressing challenges in lncRNA dis- covery and validation with its workflow that includes PrimePCR lncRNA Assays, Predesigned PrimePCR lncRNA Arrays, Custom PrimePCR lncRNA Arrays, and the novel iScript Explore One- Step RT and PreAmp Kit. The workflow offers all the tools necessary to go from experimental sample to lncRNA data analysis. Bio-Rad Laboratories Image-Analysis System The FlowCam Nano is the first nanoflow imaging particle analysis system. It features a patented oil immersion, flow- imaging technology paired with the image-analysis soft- ware VisualSpreadsheet ® to provide a comprehensive particle analysis research and development tool for parenteral drug analysis. Users have the ability to image and analyze particles ranging 300 nm to 10 μm in size, while col- lecting relative quantifications of intrinsic, extrinsic, and inher- ent particles in parenteral drugs. Finally, researchers can use morphological data to identify the structure and nature of contaminants and improve product development. Fluid Imaging Technologies Cell Culture Media OptiVERO is a chemically defined, blood-free media formulation that replaces undefined di- and tripeptide plant-derived hydrolysates with highly pure and biologically active recombinant albumin specifi- cally optimized for VERO-based virus production in both 2D and 3D cell cultures. This unique formulation enables robust VERO cell expansion and unrivaled ability to minimize cell culture variability. OptiVERO outperforms other popular VERO media and can facilitate commercial development of novel viral vaccines by enabling the production of difficult-to-express virus types. It is supplied as a concentrated frozen media and protein supplement with a base media. InVitria Bioscience High-Throughput Bioreactor The ambr ® 250 high- throughput perfusion, an automated parallel bioreactor system, has been specially designed for rapid cell culture perfusion process de- velopment to optimize production of thera- peutic antibodies. De- veloped in collaboration with major biopharma companies, it combines 12 or 24 single-use perfu- sion mini-bioreactors (100–250 mL working volume) with associated single-use perfusion components, all controlled by one automated workstation. The combination of this multi-parallel processing capacity and fully single-use perfusion vessel enables scientists to perform more perfusion culture ex- periments in a fraction of the time and cost of using traditional perfusion-enabled benchtop bioreactors. This innovation supports a range of hollow-fiber perfusion applications, enabling Design of Experiments (DoE) studies for high-cell-density process development in a Quality by Design (QbD) approach. Sartorius Stedim Biotech

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