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Thermo Scientifi c Protein Ladders Prestained The Thermo Scientifi c PageRuler and PageRuler Plus Prestained Protein Ladders are comprised of highly purifi ed recombinant proteins bound to three different chromophores for easy protein molecular weight determination. The green and orange reference bands in the PageRuler Protein Ladders provide easy orientation of the protein bands. PageRuler Prestained Protein Ladder kDa – – – – – – – – – – ~170 ~130 ~100 ~70 ~55 ~40 ~35 ~25 ~15 ~10 – – – – – – – – – – 4-20% Tris-glycine SDS-PAGE Product #s 26616, 26617 PageRuler Plus Prestained Protein Ladder kDa – ~250– – – – – – – – – ~130 ~100 ~70 ~55 ~35 ~25 ~15 ~10 – – – – – – – – 4-20% Tris-glycine SDS-PAGE Product #s 26619, 26620 2 New Protein Ladders! Multicolor Four different chromophores bound to proteins in Thermo Scientifi c Spectra Multicolor Broad Range Protein Ladders produce a brightly colored ladder with an easy-to-remember pattern. The Spectra™ Spectra Multicolor Broad Range Protein Ladder kDa – ~260 – – – – – – – – – ~140 ~100 ~70 ~40 ~35 ~25 ~15 ~10 – – – – ~50 – – – – – – 4-20% Tris-glycine SDS-PAGE Product #s 26623, 26634 – – – – – – ~40 ~25 ~15 ~10 4.6 1.7 – – – – – – 18% SP Tris-tricine SDS-PAGE SP - Small Peptide SDS - polyacrylamide gel with higher crosslinking, C=5% Product # 26628 Unstained Thermo Scientifi c PageRuler Unstained Protein Ladders are mixtures of recombinant, highly purifi ed proteins designed for accurate sizing of proteins by SDS-PAGE. The ladders resolve into clearly identifi able sharp bands when analyzed by SDS-PAGE and stained with Coomassie Blue or silver stain. Each ladder contains protein bands of greater intensity that serve as reference bands. Proteins can be detected on Western blots by staining with Ponceau S or Coomassie Blue (except for 3.4kDa and 5kDa polypeptides) or by using Strep-Tactin conjugates. PageRuler Unstained Protein Ladder kDa – – – – – – – – – – – – – – 200 150 120 100 85 70 60 50 40 30 25 20 15 10 8-16% Tris-glycine SDS-PAGE Product # 26614 – – – – – – – – – – – PageRuler Unstained Broad Range Protein Ladder kDa 250 150 100 70 50 40 30 20 15 10 5 4-20% Tris-glycine SDS-PAGE Product # 26630 Prestained NIR The PageRuler Prestained NIR Protein Ladder is a mixture of 10 proteins (11 to 250kDa) that are blue-stained and fl uor-labeled for near-IR fl uorescent visualization and protein sizing. The protein MW markers in this ladder resolve into sharp bands when analyzed by SDS-PAGE and are labeled with a fl uorescent dye for visualization with instruments equipped with near-infrared (NIR) fl uorescence detection. The bands are also directly visible because the proteins are prestained blue. PageRuler NIR Protein Ladder kDa – 250 – – – – – – – – – – 130 95 70 55 43 34 26 15 11 – – – – – – – – – 4-20% Tris-glycine SDS-PAGE Product # 26635 – – – – – – – PageRuler Unstained Low Range Protein Ladder kDa – 100 30 25 20 15 10 5 3.4 18% SP Tris-tricine SDS-PAGE Product # 26632 4-20% Tris-glycine SDS-PAGE Product # 26637 Chemiluminescent Thermo Scientifi c SuperSignal Molecular Weight Protein Ladders contain a ready-to-use stabilized mixture of eight recombinant proteins ranging in size from 20 to 150kDa. These recombinant proteins bind antibodies used in the Western blot through an IgG binding site. The protein markers can then be visualized either using appropriate substrates for enzyme-labeled antibodies or via fl uorescent dye-labeled antibodies. Use SuperSignal™ Enhanced Molecular Weight Protein Ladders with mouse monoclonals. 4-12% Bis-Tris SuperSignal Molecular Weight Protein Ladder 5µL 10µL 150kDa 100kDa 80kDa 60kDa 50kDa 40kDa 30kDa 20kDa 4-20% Tris-Glycine Product #s 84785, 84786 5µL 10µL – – – – – – – PageRuler Unstained High Range Protein Ladder kDa 250 200 – 150 120 100 85 70 60 – ~50 – – – ~35 ~25 ~20 8-16% Tris-glycine SDS-PAGE – – Cost effective The Thermo Scientifi c Pierce Prestained Protein Molecular Weight Marker is designed to monitor protein migration during SDS-polyacrylamide gel electrophoresis and monitor protein transfer onto membranes. Pierce™ Unstained Protein Molecular Weight Marker produces sharp bands on SDS-polyacrylamide gel following staining with Coomassie-based or silver stains and is recommended for accurate sizing of proteins on SDS PAGE and Western blots. Pierce Prestained Protein MW Marker kDa ~120 ~ 85 – – – – – – – Pierce Unstained Protein MW Marker kDa 116.0 66.2 45.0 35.0 25.0 18.4 14.4 8-16% Tris-glycine SDS-PAGE Protein β-galactosidase Bovine serum albumin Ovalbumin Lactate dehydrogenase REase Bsp98I β-lactoglobulin Lysozyme – – – – – – – – ~300 ~250 ~180 ~130 ~100 ~70 ~50 ~40 – – – – – – – – 4-12% Tris-glycine SDS-PAGE Product # 26625 Spectra Multicolor Low Range Protein Ladder kDa Spectra Multicolor High Range Protein Ladder kDa Multicolor High Range and Low Range Protein Ladders are designed for large and small protein analysis, respectively. thermoscientifi c.com/pierce © 2012 Thermo Fisher Scientifi c Inc. All rights reserved. These products are supplied for laboratory or manufacturing applications only. Visit our website for up-to-date prices. Facebook is a registered trademark of Facebook, Inc. All other trademarks are property of Thermo Fisher Scientifi c Inc. and its subsidiaries. Life Science Research Africa /Belgium/Europe/Middle East +32 53 85 71 84 France 0 800 50 82 15 Germany 0228 9125650 Netherlands 076 50 31 880 Switzerland 0800 56 31 40 UK 0800 252 185 1602463 09/12 Printed in the U.S. Email: perbio.euromarketing@thermofi sher.com thermoscientifi c.com/perbio For other regions, visit thermoscientifi c.com/piercedistributors USA +815-968-0747 or +800-874-3723 Customer Assistance E-mail:Pierce.CS@thermofi sher.com thermoscientifi c.com/pierce Check out our Molecular Weight Marker selection guide at thermoscientifi c.com/MW-ladder and fi nd out which Thermo Scientifi c Protein Ladder is right for you. NEW! NEW! Gel Gel Blot Gel Gel Gel Blot Visible Light Gel Near-IR Imager Gel Gel Blot Gel Gel Blot Gel Gel Blot X-ray film