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16 | JULY 2016 | GENengnews.com | Genetic Engineering & Biotechnology News Peter Rezk Microplates are the workhorses of the lab. They are used in almost every application, from sample collection and preparations to measuring the faint signal of a low abundant biomarker in a blood sample. In this tuto- rial, we will examine the suitability of using AntiBIND family of microplates in protein research applications such as protein storage, high throughput protein discovery, biochemi- cal analysis via chromatographically applica- tion LC/MS. Starting with the Right Raw Materials When it comes to lab consumables such as microplates, micro tubes, and other dispos- able containers, the plastic of choice is poly- propylene (PP). PP is rugged and resistant to many chemicals, acids, and bases with a melt- ing point of anywhere from 130 °C (266 °F) up to 171 °C (340 °F). An essential aspect of manufacturing these consumables is the selec- tion of the right raw materials. One of the best suited PP grade for lab consumables is homo- polymer PP grade using metallocene catalysts. These resins have improved optical and pro- cessing characteristics, combined with excep- tional purity, and minimal additives that can be leached into the solution. Unfortunately, many companies use injection molding grades of PP with high impurities and add to the resin other extractables additives to increase manufacturing output at lower costs. These leachate compounds can be grouped into groups such as mold releasing agents, poly- mer fow improvers, surface coating, and or other materials that allow for a modifcation in the plastic outer properties. The leaching of these additives may interfere with solute and or proteins in solution jeopardizing sample in- tegrity and biochemical results. The negative impact of this leaching phenomenon is well reported and observed in long term sample storage applications, solutions containing sol- vents such as methanol, DMSO, or detergents such as EDTA and or high/low pH solutions. We tested AntiBIND as well as fve other treated and untreated microplates available in the market to understand the extractions profle of these plates and the different chal- lenges they may pose to the researcher. The plates were cryogenically ground in a freezer mill to produce a high surface area and then extracted with chloroform for two hours. After fltration, extracts were analyzed by GC-MS and LC-MS. Additional extraction experiments conducted via regular incuba- AntiBIND: Ultimate Recovery of Your Valuable Proteins at Low Concentrations Engineering the Right Microplate for Tomorrow's Therapeutics DRUG DISCOVERY Tutorial VIAFLO II VOYAGER II ASSIST VIAFLO 96 I 384 EVOLVE Manual Pipette Unlike traditional pipettes which utilize a single rotating plunger to set volumes, the EVOLVE features three dials for setting each individual volume digit. This revolutionary approach allows users to set volumes more than ten times faster. SET VOLUMES IN THE BLINK OF AN EYE INSTEAD OF A TWIST OF THE WRIST www.integra-biosciences.com Visit us at AACC 2016 Booth # 3741 August 2 - 4 Philadelphia Figure 1. AntiBIND 96 deep well plate, volume 0.5 mL with conical well design Figure 3. The use of AntiBIND microplates has increased the percent recovery rate of sample by 50– 300% over best-in-class low protein binding plates. The trend of increased sample recovery rate by using AntiBIND microplates over best-in-class microplates was observed with diferent proteins at diferent concentrations. (Data will be available in AntiBIND Tech. Note I.) When standard polypropylene plates are used at these low concentrations, the recovery rate after 24 hours was almost zero. The superior performance of AntiBIND microplates over best- in-class low protein binding plates and standard polypropylene plates across a range of proteins will allow for analytical reliability, which ultim- ately leads to cost savings, improved accuracy, and efcient allocation of valuable lab resources. Figure 2. AntiBIND plates do not show extractables species, whereas the competitive plates do leach components that interfere with detection and analysis. The stripping of the low binding capability of the competitors' plates will decrease its low binding capability and allow for greater protein adsorption to the wall of the plate.