Transfer proteins faster and easier
with Thermo Scientifc Pierce G2 Fast Blotter
touch. transfer. time. tank.
(no tank needed)
• Easy-touch programming
– access pre-programmed transfer
methods or easily create, run and save
custom transfer methods
• Easy-to-use color LCD menu
touchscreen interface
– quick programming of transfer time
based on the number and size of gels
• Integrated power supply
– allows consistent high effciency
protein transfer
• Excellent transfer effciency – transfer low,
medium and high MW proteins (10-300kDa) equivalent to
or better than conventional semi-dry or wet transfer methods
Low
(<25kDa)
Mid
(25-150kDa)
High
(>150kDa)
Pierce Precise Protein
Gel (Tris-glycine)
NuPage™ 4-12%
Bis-Tris Gel
NuPage 4-12%
Bis-Tris Gel
Membrane
Nitrocellulose
Nitrocellulose
PVDF
Target protein
and size
Cyclophilin B
(21kDa)
PLK-1 (67kDa)
mTOR (289kDa)
• Fast – transfer proteins in 5–10 minutes
Molecular
weight range
Gel type
Efficient protein transfer in 10 minutes. GST-PI3K-SH2HA (37kDa) was expressed in E. coli and purifed with
varying volumes of Thermo Scientifc™ Anti-HA Magnetic
Beads (Product # 88836). Resulting samples were prepared
for SDS-PAGE and electrophoresed. Membranes were
probed with anti-HA antibody followed by anti-mouse HRP.
The blots were incubated in Thermo Scientifc™ SuperSignal™
West Femto Substrate (Product # 34096). The resulting
blots were simultaneously imaged using the Thermo
Scientifc™ MYECL™ Imager (Product # 62236), and then
band densitometry was determined using Thermo Scientifc™
™
MYImageAnalysis Software (Product # 62237).
Check out the experimental protocol video on
thermoscientifc.com/g2blotter2
• Convenient – simultaneously transfer up to four
mini-sized gels or two midi-sized gels
Thermo Scientifc™ Pierce™ 1-Step Transfer Buffer
• Safe and environmentally friendly
– Pierce 1-Step Transfer Buffer contains no alcohol
• Economical – transfer up to 20 mini-sized or 10 midi-sized
gels with one 1L bottle. No special consumables required; simply
use Western blotting flter paper and membrane of choice
Ordering Information
Product # Description
62288SPCL Pierce G2 Fast Blotter
Suffcient for semi-dry transfer of proteins from polyacrylamide gels to
nitrocellulose or PVDF membranes.
Includes: Pierce G2 Fast Blotter Control Unit, 1 device
Pierce G2 Fast Blotter Cassette, 1 device
Power Cord with C/13 Connector, 1 device
Western Blot Roller, 1 device
Quick Start Guide
Check out our special offer at
thermoscientifc.com/g2blotter2
thermoscientifc.com/pierce
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countries. Please consult your local sales representative for details.
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UK 0800 252 185
1602671 08/13 Printed in the U.S.
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thermoscientifc.com/pierce
For other regions, visit
thermoscientifc.com/piercedistributors
USA +815-968-0747 or +800-874-3723
Customer Assistance
E-mail:Pierce.CS@thermofsher.com
thermoscientifc.com/pierce
• Hassle-free – the
classical overnight tank
transfer method is history!